16S rDNA Genotyping of Lactic Acid Bacteria Using PCR-RFLP Analysis
نویسندگان
چکیده
منابع مشابه
16S rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae.
The 16S rDNA genotypes among the family Vibrionaceae were determined using PCR/RFLP analysis. Five tetrameric restriction enzymes (HhaI, DdeI, RsaI, Sau3AI and MspI) were used for RFLP analysis and adequate numbers of informative bands were obtained from each enzyme. Twenty-seven genotypes were obtained from 49 type and reference strains including 35 species. Nineteen species could be assigned ...
متن کاملIdentification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.
DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
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The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry. Despite efforts to control this disease, the high frequency of new epizootic events indicate that the vaccine and antibiotics treatments have limited effectiveness, therefore the preve...
متن کاملمطالعه تنوع ژنتیک باکتری‌های سینوریزوبیوم با استفاده از تکنیک PCR / RFLP 16S-23S rDNA
It is important to investigate the genetic diversity and evaluate symbiotic effectiveness of the indigenous rhizobial population. It helps understand the responses of indigenous isolates to different rhizobial inoculants. In spite of the importance of bacterial diversity, there are a few scientific reports about it in Iranian soils. Genetic diversity of 150 isolates of Sinorhizobium isolated ...
متن کاملمطالعه تنوع ژنتیک باکتری‌های سینوریزوبیوم با استفاده از تکنیک PCR / RFLP 16S-23S rDNA
It is important to investigate the genetic diversity and evaluate symbiotic effectiveness of the indigenous rhizobial population. It helps understand the responses of indigenous isolates to different rhizobial inoculants. In spite of the importance of bacterial diversity, there are a few scientific reports about it in Iranian soils. Genetic diversity of 150 isolates of Sinorhizobium isolated ...
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ژورنال
عنوان ژورنال: NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
سال: 2017
ISSN: 1341-027X,1881-6681
DOI: 10.3136/nskkk.64.355